|
| Status |
Public on Jan 02, 2017 |
| Title |
filaggrin mutaion [A2483-09] |
| Sample type |
RNA |
| |
|
| Source name |
filaggrin mutation
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin, ear, FLG mut
condition: LS
genotype: filaggrin mutation
Sex: female
age: 26W
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The normalization and summarization was carried out in R, using:
- rma {affy}
- normalize.quantiles.robust {preprocessCore}
Versions: preprocessCore_1.32.0 ; affy_1.48.0
|
| |
|
| Submission date |
May 04, 2016 |
| Last update date |
Jan 02, 2017 |
| Contact name |
David Adrian Ewald |
| E-mail(s) |
ewald@cbs.dtu.dk
|
| Organization name |
Technical University of Denmark
|
| Department |
Department of Systems Biology
|
| Lab |
Regulatory Genomics
|
| Street address |
Building 208
|
| City |
Kongens Lyngby |
| ZIP/Postal code |
DK-2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE81119 |
Major differences between human atopic dermatitis and murine models as determined by global genomic profiling |
|
