|
| Status |
Public on Oct 24, 2017 |
| Title |
H2A.Z |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
H2A.Z ChIP DNA from cells harvested 4h after meiosis induction
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
antibody: H2A
|
| Growth protocol |
Cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested four hours after the induction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except for using anti-H2A.Z antibodies (Abcam).
|
| Label |
biotin
|
| Label protocol |
Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83)
|
| |
|
| Channel 2 |
| Source name |
Input DNA from cells harvested 4h after meiosis induction
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
antibody: input
|
| Growth protocol |
Cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested four hours after the induction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except for using anti-H2A.Z antibodies (Abcam).
|
| Label |
biotin
|
| Label protocol |
Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83)
|
| |
|
| |
| Hybridization protocol |
16 h at 45C using a hybridization oven 640 (affymetrix) as described in the Affymetrix Chromatin Immunoprecipitation Assay Protocol (P/N 702238)
|
| Scan protocol |
Fluidics station 400 protocol EukGE-WS2v5 (affymetrix) was performed to wash and stain arrays. The arrays were scanned on GeneChip Scanner 3000 7G (affymetrix).
|
| Description |
H2A.Z / WCE, meiosis 4h
|
| Data processing |
Data were quantile normalized and smoothed with Affymetrix Tiling Analysis Software
|
| |
|
| Submission date |
May 23, 2016 |
| Last update date |
Oct 24, 2017 |
| Contact name |
Kazuto Kugou |
| E-mail(s) |
kkugou@kazusa.or.jp
|
| Organization name |
Kazusa DNA Research Institute
|
| Department |
Department of Frontier Research
|
| Lab |
Laboratory of Cell Engineering
|
| Street address |
2-6-7 KazusaKamatatri
|
| City |
Kisarazu |
| State/province |
Chiba |
| ZIP/Postal code |
292-0818 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7715 |
| Series (2) |
| GSE81776 |
The histone variant H2A.Z promotes initiation of meiotic recombination (ChIP) |
| GSE81777 |
The histone variant H2A.Z promotes initiation of meiotic recombination |
|
