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Sample GSM2176549 Query DataSets for GSM2176549
Status Public on Feb 14, 2018
Title Cre^(ER)/Rptor^(fl/+) pro-B cells untreated replicate 2
Sample type RNA
 
Source name Untreated pro-B cells from Cre^(ER)/Rptor^(fl/+) mouse
Organism Mus musculus
Characteristics cell population: B220+CD43+IgM- pro-B
strain: C57BL/6 Cre^(ER)/Rptor^(fl/+)
Treatment protocol Pro-B cells from tamoxifen-treated Cre^(ER)/Rptor^(fl/+) and Cre^(ER)/Rptor^(fl/fl) mice were cultured in the presence or absence of 25 ng/ml IL-7 for 24h.
Growth protocol Lymphocytes were isolated from bone marrow of mice, and pro-B cells (B220+CD43+IgM-) were sorted on a MoFlow (Beckman-Coulter) or Reflection (i-Cyt).
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen's RNAeasy kit, with on-column DNase digestion included.
Label biotin
Label protocol One to five nanograms of total RNA were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
 
Hybridization protocol Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Mouse Gene 2.0 ST GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
Data processing Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.6
 
Submission date May 24, 2016
Last update date Feb 14, 2018
Contact name Geoffrey Neale
E-mail(s) geoffrey.neale@stjude.org
Organization name St Jude Childrens Research Hospital
Department Hartwell Center
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL16570
Series (1)
GSE81844 B cell development requires discrete regulation by PTEN-PI3K and mTOR pathways and the intricate interplay with IL-7 r-Stat5 signaling

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
17200001 4.97114
17200003 5.78016
17200005 2.82943
17200007 3.88299
17200009 5.10688
17200011 5.17421
17200013 6.12617
17200015 5.01585
17200017 4.29703
17200019 3.1339
17200021 3.63056
17200023 5.60962
17200025 6.04641
17200027 6.0343
17200029 3.40174
17200031 3.23182
17200033 4.14875
17200035 4.48927
17200037 5.89076
17200039 3.30554

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.




Supplementary file Size Download File type/resource
GSM2176549_HZR461_482679_mogene2st.CEL.gz 8.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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