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Status |
Public on May 27, 2016 |
Title |
MCF-10A_Control, biological rep1 |
Sample type |
RNA |
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Source name |
MCF-10A transfected with a control vector
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Organism |
Homo sapiens |
Characteristics |
strain: MCF-10A mammary epithelial cell line
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Treatment protocol |
SNAI1 or a vector control was introduced into MCF-10A cells, respectively.
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Growth protocol |
MCF-10A mammary epithelial cells were cultured according to the manufacturer's instructions.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
|
Label protocol |
Biotinylated miRNA were prepared using a FlashTag™ Biotin RNA Labeling Kit for Affymetrix® GeneChip® miRNA Arrays (Genisphere, FT30AFYB) from 1ug total RNA.
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Hybridization protocol |
Biotinylated miRNA prepared from 1ug total RNA were hybridized for 16h at 48℃ on GeneChip miRNA 2.0 Array. GeneChips were washed and stained in the Affymetrix ® Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
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Description |
microRNA expression data from MCF-10A transfected with a vector control
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Data processing |
The data were analyzed with Affymetrix® GeneChip® Command Console™ 1.1 using Affymetrix default analysis settings and global scaling as normalization method.
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Submission date |
May 26, 2016 |
Last update date |
May 27, 2016 |
Contact name |
Zhihua Liu |
E-mail(s) |
liuzh@cicams.ac.cn
|
Phone |
8610-67723789
|
Organization name |
National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
|
Street address |
NO.17 Nanli, Panjiayuan Road, Chaoyang District
|
City |
Beijing |
ZIP/Postal code |
100021 |
Country |
China |
|
|
Platform ID |
GPL14613 |
Series (2) |
GSE81930 |
SNAI1 overexpression effect on MCF-10A mammary epithelial cell line (miRNA) |
GSE81931 |
SNAI1 overexpression effect on MCF-10A mammary epithelial cell line |
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