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Sample GSM2177878 Query DataSets for GSM2177878
Status Public on May 27, 2016
Title MCF-10A_Control, biological rep1
Sample type RNA
 
Source name MCF-10A transfected with a control vector
Organism Homo sapiens
Characteristics strain: MCF-10A mammary epithelial cell line
Treatment protocol SNAI1 or a vector control was introduced into MCF-10A cells, respectively.
Growth protocol MCF-10A mammary epithelial cells were cultured according to the manufacturer's instructions.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated miRNA were prepared using a FlashTag™ Biotin RNA Labeling Kit for Affymetrix® GeneChip® miRNA Arrays (Genisphere, FT30AFYB) from 1ug total RNA.
 
Hybridization protocol Biotinylated miRNA prepared from 1ug total RNA were hybridized for 16h at 48℃ on GeneChip miRNA 2.0 Array. GeneChips were washed and stained in the Affymetrix ® Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
Description microRNA expression data from MCF-10A transfected with a vector control
Data processing The data were analyzed with Affymetrix® GeneChip® Command Console™ 1.1 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date May 26, 2016
Last update date May 27, 2016
Contact name Zhihua Liu
E-mail(s) liuzh@cicams.ac.cn
Phone 8610-67723789
Organization name National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
Street address NO.17 Nanli, Panjiayuan Road, Chaoyang District
City Beijing
ZIP/Postal code 100021
Country China
 
Platform ID GPL14613
Series (2)
GSE81930 SNAI1 overexpression effect on MCF-10A mammary epithelial cell line (miRNA)
GSE81931 SNAI1 overexpression effect on MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
ACA10_s_st 71.29834
ACA12_st 29.56075
ACA13_st 85.95314
ACA15_s_st 61.45456
ACA15_x_st 39.65962
ACA16_st 77.65865
ACA16_x_st 50.03898
ACA18_x_st 224.9531
ACA20_st 41.62838
ACA20_x_st 30.43175
ACA21_st 32.37663
ACA23_st 29.63487
ACA24_s_st 126.9307
ACA24_x_st 327.1478
ACA25_st 24.57326
ACA25_x_st 49.64352
ACA26_st 51.99232
ACA27_x_st 28.76616
ACA3_st 113.507
ACA32_st 23.24854

Total number of rows: 622

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM2177878_MCF-10A_C1_miRNA-2_0_.CEL.gz 688.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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