|
| Status |
Public on Jun 03, 2016 |
| Title |
Dermal fibroblast from donor, KA |
| Sample type |
RNA |
| |
|
| Source name |
Dermal fibroblast from donor
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: KA
cell type: Dermal fibroblast (DF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 °C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
DF(KA)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500. Normalized data matrices available on the series record contain MAS 5.0 signal. Normalizations were performed on sample subsets.
|
| |
|
| Submission date |
Jun 02, 2016 |
| Last update date |
Jun 03, 2016 |
| Contact name |
Koki Fujimori |
| E-mail(s) |
koh0909@a3.keio.jp
|
| Organization name |
Keio University
|
| Department |
Physiology
|
| Street address |
35 Shinanomachi
|
| City |
Sinjuku-ku, Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE82159 |
Expression data of lymphoblastoid B-cell line (LCL), T-cell, dermal fibroblast (DF), and iPSCs established from them. |
|
