|
| Status |
Public on Aug 29, 2017 |
| Title |
WT_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
WT Treg cells from mixed BM chimeras
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mixed bone marrow (BM) chimeras
genotype/variation: wild type
cell type: Treg cells
|
| Treatment protocol |
Sorted Treg cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
| Growth protocol |
Treg cells were sorted from mixed BM chimeras without additional culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
One to five nanograms of total RNA were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Mouse Gene 2.0 ST GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Description |
kyr008
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.6
|
| |
|
| Submission date |
Jun 07, 2016 |
| Last update date |
Aug 29, 2017 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE83088 |
Transcription profile of WT and LKB1 (encoded by the gene Stk11)-deficient Treg cells |
|
