|
| Status |
Public on Nov 30, 2016 |
| Title |
DsBgDicer-1_1 |
| Sample type |
SRA |
| |
|
| Source name |
DsBgDicer-1
|
| Organism |
Blattella germanica |
| Characteristics |
developmental stage: Nymph 6 day 2
genotype/variation: DsBgDicer-1
tissue: Whole body
|
| Treatment protocol |
5-day-old fifth instar female nymphs were treated with a 3µg of dsBgDicer-1 twice, first just after the emergency and secondly 3 days later, and were dissected 2 days after molting to the last (sixth) nymphal instar (Lozano et al 2015). As control we followed the same protocol but injecting dsPolyhedrin.
|
| Growth protocol |
Blattella germanica specimens used for the whole transcriptome analysis were obtained from a population reared at the Institut de Biologia Evolutiva (CSIC-UPF). Conditions were 70% humidity at 30 ºC and a commercial dog food diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kit (Qiagen)
NEBNext® Small RNA Library Prep Set for Illumina®
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
T1
|
| Data processing |
Quality control with FastQC
Read filtering and trimming with Trimmomatic
Reads mapping with Bowtie
miRNA counts with FeatureCounts
Genome_build: v1 (Bger_1.0)
Supplementary_files_format_and_content: [.csv] file reports miRNA counts
|
| |
|
| Submission date |
Jun 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Guillem Ylla |
| E-mail(s) |
guillem.ylla@ibe.upf-csic.es
|
| Organization name |
Institute of Evolutionary Biology
|
| Lab |
Belles & Piulachs Labs
|
| Street address |
Passeig Marítim de la Barceloneta, 37
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL22010 |
| Series (1) |
| GSE83339 |
The microRNA toolkit of insects |
|
| Relations |
| BioSample |
SAMN05244995 |
| SRA |
SRX1844454 |
