|
| Status |
Public on Jun 20, 2019 |
| Title |
panicle of heat tolerant line 252 under high temperature, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Rice panicle under high temperature treatment
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: Panicle
|
| Treatment protocol |
High temperature treatment( 37 ℃ day/30 ℃ night cycle for 5 d),optimal temperature treatment(30 ℃day/ 25 ℃ night cycle) .
|
| Growth protocol |
panicles were collected,frozen in liquid nitrogen immediately,and stored at -80 ℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45 ℃ on Affymetrix GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip® Scanner 3000 with default settings.
|
| Description |
Gene expression data from panicle under high temperature treatment
|
| Data processing |
Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software 11.0,The gene classification based on gene ontology was conducted as previously described(Hobo et al. 2008). The pathway analysis was performed with MapMan
|
| |
|
| Submission date |
Jun 20, 2016 |
| Last update date |
Jun 20, 2019 |
| Contact name |
Xianwen Zhang |
| E-mail(s) |
xianwenzhang@hunau.edu.cn
|
| Phone |
08613875856138
|
| Organization name |
Hunan Agricultural University
|
| Street address |
1 Nongda Road Furong district
|
| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410128 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE83542 |
Expression data from panicle in heat tolerant and heat susceptible rice under high temperature stress |
|
