|
| Status |
Public on Jan 01, 2023 |
| Title |
Bone marrow LT-HSC, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
CD48-CD150+CD34-Lin-Sca1+Kit+ LT-HSCs sorted from C57/Bl6 mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
tissue: bone marrow
cell type: LT-HSC
age: 8 week
cell markers: CD48-CD150+CD34-Lin-Sca1+Kit+
|
| Treatment protocol |
Cells were sorted from mouse whole bone marrow, treated with DMSO for 2 hours, then transplanted into primary recipients. Cells were isolated from primary recipients one year later then transplanted into secondary recipients, without further treatment. After 3 months, cells were isolated from secondary recipients and used for total RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Amplification and library preparation was performed using the Ovation Pico WTA System V2 and Encore Biotin Module labeling.
|
| |
|
| Hybridization protocol |
Samples were hybridized to Mouse Gene 2.0 ST Arrays (Affymetrix) and scanned at the Microarray Core Facility at the IDDRC of Boston Children's Hospital and Harvard Medical School.
|
| Scan protocol |
Scanning was done per manufacturer's instructions.
|
| Description |
Gene expression of sorted mouse bone marrow LT-HSC
No treatment
|
| Data processing |
Raw data were processed using the Affymetrix Command Console Software to determine RMA-normalized expression values (log2) and used to identify differences in gene expression between GFP (+) and (-) samples.
|
| |
|
| Submission date |
Jul 07, 2016 |
| Last update date |
Jan 01, 2023 |
| Contact name |
Leonard Zon |
| E-mail(s) |
zon@enders.tch.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Department |
Oncology/Hematology
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE84127 |
Expression analysis of Chd7 in mouse hematopoietic stem and progenitor cells [microarray] |
| GSE84131 |
CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation |
|
