|
| Status |
Public on Jun 30, 2017 |
| Title |
B_RNA-seq_AkataZta+I-BET_Replicate1 |
| Sample type |
SRA |
| |
|
| Source name |
Akata-Zta (EBV+ Burkitt lymphoma cell line)
|
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Characteristics |
cell line: Akata-Zta
pathogen: Epstein-Barr virus
1h pretreatment: I-BET
1 day treatment: vehicle
|
| Treatment protocol |
Log phase cultures were pretreated for 1 hour with 1 μM JQ1 (EMD Millipore), I-BET/GSK525762A (EMD Millipore), or vehicle, then treated for 1 day with 500 ng/mL doxycycline (Sigma-Aldrich) or vehicle
|
| Growth protocol |
Akata-Zta cells were maintained in RPMI-1640 with 25 mM HEPES and 2 g/L NaHCO3 in 5% CO2 with 10% (v/v) Tet System Approved fetal bovine serum (Clontech)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and cDNA was prepared as described in the paper
RNA-seq libraries were prepared as described in the paper
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
50 bp reads were mapped using Bowtie to an index containing both the human hg19 and EBV reference [GenBank ID: NC_007605.1] genomes.
Parameters allowed for up to two mismatches and only considered reads that mapped to a unique sequence.
The number of hits at each base was counted and then normalized per million mapped reads.
Formatted for uploading to Integrative Genomics Viewer (www.broadinstitute.org/igv/)
Genome_build: Index contains both the human hg19 and EBV [GenBank ID: NC_007605.1] reference genomes
Supplementary_files_format_and_content: Wigs are formatted for IGV. Genome file chrEBV(B95_8_Raji).fa is available from http://www.flemingtonlab.com/rnaseq.html. Wig columns are chromosome, start, stop, counts per million mapped reads.
|
| |
|
| Submission date |
Jul 10, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
JJ Miranda |
| E-mail(s) |
jmiranda@barnard.edu
|
| Organization name |
Barnard College, Columbia University
|
| Street address |
3009 Broadway
|
| City |
New York |
| ZIP/Postal code |
10027 |
| Country |
USA |
| |
|
| Platform ID |
GPL22132 |
| Series (2) |
| GSE84211 |
BET inhibitors suppress lytic DNA replication [Akata-Zta RNA-seq] |
| GSE84214 |
BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps |
|
| Relations |
| BioSample |
SAMN05370874 |
| SRA |
SRX1923385 |
