|
| Status |
Public on May 01, 2017 |
| Title |
rCNT aspiration 4days + 24h M3_4d24h |
| Sample type |
RNA |
| |
|
| Source name |
M3 4d 24h
|
| Organism |
Mus musculus |
| Characteristics |
exposure: 10 ug of rCNT in 50 ul of PBS
exposure method: aspiration
time: 4d + 24h
|
| Treatment protocol |
mice lung tissue samples collected 24h after final exposure. Tissue samples stored in RNAlater in -80ºC
|
| Growth protocol |
Female C57BL/6 mice (7-8 weeks old) were housed in groups of four in stainless steel cages, bedded with aspen chip and were provided with standard mouse chow diet and tab water ad lib. 12 h dark/light cycle was used in the animal room temperature being 20-21 degree celcius and humidity of 40-45 %.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation from the lung samples was done with TRIsure (phenol-chloroform extraction) and the quantity and purity was determined by NanoDrop spectrophotometer. Further quality check of the total RNA was performed with the Agilent BioAnalyzer, according to the manufacturer's recommendations.
|
| Label |
Cy5
|
| Label protocol |
Samples were amplified using T7 RNA polymerase method and the cRNAs were further labeled with Cy3 and Cy5 dye, according to the manufacturer's recommendations.
|
| |
|
| Hybridization protocol |
Labeled cRNAs were hybridized to Agilent 2-color 60-mer oligo arrays, , according to the manufacturer's recommendations.
|
| Scan protocol |
Slides were washed and scanned with Agilent Microarray scanner G2505C, according to the manufacturer's recommendations.
|
| Data processing |
Raw intensity values were obtained from Agilent Feature Extraction software and quality checked according to Agilent standard procedures. The median foreground intensities were imported into R software and analyzed with BioConductor package Limma.
|
| |
|
| Submission date |
Aug 16, 2016 |
| Last update date |
May 01, 2017 |
| Contact name |
Dario Greco |
| E-mail(s) |
dario.greco@tuni.fi
|
| Organization name |
Tampere University
|
| Department |
Faculty of Medicine and Health Technology
|
| Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
|
| Street address |
Arvo ylpön Katu 34
|
| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE85711 |
Inhalation and oropharyngeal aspiration exposure to rod-like carbon nanotubes induces highly similar airway inflammation and biological pathways in mouse lungs |
|
