|
| Status |
Public on Mar 24, 2017 |
| Title |
ST486-EV-IP1 |
| Sample type |
RNA |
| |
|
| Source name |
ST486 cell line with empty vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ST486
cell type: Burkitt lymphoma cell line
transduced with: empty vector
ip with: AGO2 ab (Clone 2E12-1C9)
molecule subtype: AGO2-IP RNA
|
| Treatment protocol |
Cells were transduced with empty lentiviral pCDH-EF1α vector, GFP+ cell were sorted and subjected to imunoprecipitation using AGO2 ab (Clone 2E12-1C9)
|
| Growth protocol |
RPMI-1640 medium supplemented with 100U/ml penicillin, 0.1mg/ml streptomycin, 2mM ultraglutamine and 10% (DG75) or 20% (ST486) Fetal Calf Serum, 37⁰C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Micro/Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Cy3 labelled using 50-100ng total RNA in combination with the LowInput QuickAmp Labelling Kit, Cyanine 3 CTP Dye Pack and RNA Spike-In kit (all Agilent Technologies).
|
| |
|
| Hybridization protocol |
Each Cy-3 sample was mixed with the same amount of a Cy5-labeled sample, which was non-relevant for this study, and hybridized at 65oC overnight on Agilent-050524 SurePrint G3 Custom Human 8x60K Microarrays.
|
| Scan protocol |
Agilent DNA Microarray Scanner, Agilent Feature Extraction software version 10.7.3.1.
|
| Description |
mRNA and lncRNA expression analysis_ST486-EV_AGO2IP fraction_1
Cy5 signals in raw data files represent unrelated samples
|
| Data processing |
GeneSpring GX 12.5 software (Agilent Technologies); quantile normalization without base line transformation.
|
| |
|
| Submission date |
Sep 05, 2016 |
| Last update date |
Mar 24, 2017 |
| Contact name |
Agnieszka Dzikiewicz-Krawczyk |
| E-mail(s) |
krawczyk@man.poznan.pl
|
| Phone |
+48616579219
|
| Organization name |
Institute of Human Genetics, Polish Academy of Sciences
|
| Street address |
Strzeszynska 32
|
| City |
Poznan |
| ZIP/Postal code |
60-479 |
| Country |
Poland |
| |
|
| Platform ID |
GPL18641 |
| Series (1) |
| GSE86432 |
Gene expression analysis to identify target genes of miR-150 in Burkitt lymphma cell lines |
|
