|
| Status |
Public on Mar 30, 2017 |
| Title |
Ppara KO mouse #3 |
| Sample type |
RNA |
| |
|
| Source name |
C57Bl/6 adult mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
background strain: C57Bl/6
gender: female
developmental stage: adult - 12-14 weeks
genotype: Ppara KO
|
| Growth protocol |
Mice were housed in a temperature-controlled room (22–24°C) with a relative humidity of 36%–80%, and 12-hour light/12-hour dark cycles. Animal studies were performed in compliance with European Community specifications regarding the use of laboratory animals and approved by the Nord-Pas de Calais Ethical Committee for animal use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). Total RNA was checked for quantity and quality using the Agilent 2100 Bioanalyzer (Agilent Biotechnologies)
|
| Label |
biotin
|
| Label protocol |
RNA were processed using Affymetrix GeneChip® WT PLUS Reagent Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on MoGene-2_0-st Chip. GeneChips were washed and stained in the Affymetrix Fluidics
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G and Affymetrix GeneChip Command Console software (AGCC)
|
| Description |
Transcriptomic liver of Ppara kO mouse
|
| Data processing |
RMA and Quantile Normalization with median polish using Partek. The data obtained are log2-transformed
|
| |
|
| Submission date |
Oct 03, 2016 |
| Last update date |
Mar 31, 2017 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
jerome.eeckhoute@inserm.fr
|
| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
|
| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
|
| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE87567 |
Transcriptomic analysis of the the liver of Ppara KO mice |
|
