|
Status |
Public on Dec 20, 2007 |
Title |
MM0278-001_StyI |
Sample type |
genomic |
|
|
Source name |
lymphoblast or blood
|
Organism |
Homo sapiens |
Characteristics |
Family ID: MM0278-001 DNA ID: 57786 Gender: F Family Type: SPX Individual Type: parents
|
Treatment protocol |
Not Applicable
|
Growth protocol |
Not Applicable
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from blood or lymphoblast using a standard phenol/chloroform method. 500ng of genomic DNA was used for subsequent PCR complexity reduction (GeneChip® Mapping 500K Assay Manual, 2005, Affymetrix)
|
Label |
biotin
|
Label protocol |
Biotinylated genomic DNA were prepared according to the standard Affymetrix protocol from 90 ug PCR product (GeneChip® Mapping 500K Assay Manual, 2005, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 90 ug of genomic DNA were hybridized for 16 hr at 49C on either the GeneChip® Mapping NspI or StyI chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G.
|
Description |
SNP data from trio simplex family
|
Data processing |
The data were processed using GCOS software. Genotypes were analyzed with GTYPE using Affymetrix defaults.
|
|
|
Submission date |
Oct 03, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Christian R. Marshall |
URL |
http://www.tcag.ca/
|
Organization name |
The Hospital for Sick Children
|
Department |
Genetics & Genome Biology
|
Street address |
101 College St.
|
City |
Toronto |
State/province |
ON |
ZIP/Postal code |
M5G 1L7 |
Country |
Canada |
|
|
Platform ID |
GPL3720 |
Series (1) |
GSE9222 |
Structural Variation of Chromosomes in Autism Spectrum Disorder. |
|