|
| Status |
Public on Aug 17, 2017 |
| Title |
Metastases, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
metastatic ccRCC tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney
cell type: metastatic clear cell renal cell carcinoma (ccRCC)
age: 72y
|
| Treatment protocol |
ccRCC tissues were collected from patients. These tissues were flash frozen in liquid nitrogen immediately after surgery and subsequently stored at -80℃. All the resected nodules or metastatic masses were identified as renal tumor by pathologic examination. No patients received anticancer treatments before surgery in this study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (GeneChip® Eukaryotic Double Strand Whole Transcript Protocol, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, ~20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Glue Grant Human Transcriptome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from metastatic ccRCC tissue.
|
| Data processing |
The data were analyzed with log scale robust multi-array analysis (RMA) using Affymetrix Power Tools as normalization method.
|
| |
|
| Submission date |
Oct 19, 2016 |
| Last update date |
Aug 17, 2017 |
| Contact name |
Jia-kuan Li |
| E-mail(s) |
jiakuanli@yeah.net
|
| Phone |
+8615618059211
|
| Organization name |
Changzheng Hospital
|
| Department |
Urology
|
| Street address |
Fengyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL16238 |
| Series (1) |
| GSE88948 |
LncRNA from metastatic clear cell renal cell carcinoma (ccRCC) compared with non-metastatic clear cell renal carcinoma |
|
