|
| Status |
Public on Jan 19, 2017 |
| Title |
Female wild type mouse_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Female wild type mouse_replicate1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole liver
gender: Female
age: 10 to 11 weeks
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver tissues with Trizol reagents following manufacture's protocol. Extracted RNA quality was checked by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA was prepared from 0.1 ug RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions. cRNA was quantified with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
The labelled cRNA was hybridized to the Agilent Whole Mouse Genome Microarray v2 8×60k following manufacture's protocol.
|
| Scan protocol |
Arrays were scanned using Agilent G2565A Microarray Scanner System.
|
| Description |
Gene expression in female wild type mouse liver
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1.3.1 (Agilent Technology) using default parameters.
|
| |
|
| Submission date |
Oct 24, 2016 |
| Last update date |
Jan 19, 2017 |
| Contact name |
Akihide Kamiya |
| E-mail(s) |
kamiyaa@tokai-u.jp
|
| Organization name |
Tokai University
|
| Street address |
143 Shimokasuya
|
| City |
Isehara |
| State/province |
Kanagawa |
| ZIP/Postal code |
259-1193 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE89091 |
The effect of Bcl6 on the liver sex difference |
|
