|
| Status |
Public on Oct 20, 2007 |
| Title |
breast nontumor part_N_rep1_1591 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Nontumor part (also called normal(N))
|
| Organism |
Homo sapiens |
| Characteristics |
er/pr/her status: NA
gender: female
tissue: non-tumor part of breast
|
| Treatment protocol |
The surgical removed tumors and non-tumor part of breast specimens were excised, collected and snap-frozen in liquid nitrogen immediately before RNA extraction.
|
| Growth protocol |
The fresh samples used for this study were immediately frozen by liquid nitrogen without any additional growth procedure applied before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent combined with Rneasy mini kit
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit, protocol version 2.0
|
| |
|
| Channel 2 |
| Source name |
Stratagene's human common reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Stratagene's human common reference RNA
|
| Treatment protocol |
The surgical removed tumors and non-tumor part of breast specimens were excised, collected and snap-frozen in liquid nitrogen immediately before RNA extraction.
|
| Growth protocol |
The fresh samples used for this study were immediately frozen by liquid nitrogen without any additional growth procedure applied before RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent combined with Rneasy mini kit
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit, protocol version 2.0
|
| |
|
| |
| Hybridization protocol |
Agilent Oligo Microarray Kit, hybridization protocol version 5.0
|
| Scan protocol |
Agilent Oligo Microarray Kit, hybridization protocol version 5.0 with Agilent scanner and Feature Extraction 6.0.
|
| Description |
Hybridized with Buffer1
|
| Data processing |
Agilent Two-Color Microarray-Based Gene Expression Analysis, version 4.0
|
| |
|
| Submission date |
Oct 12, 2007 |
| Last update date |
Oct 06, 2011 |
| Contact name |
Hsin-Chieh Yao |
| E-mail(s) |
r95524023@ntu.edu.tw
|
| Organization name |
National Taiwan University
|
| Department |
CHE
|
| Lab |
PSE
|
| Street address |
1, Rd. Roosevelt, Sec. 4
|
| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL887 |
| Series (2) |
| GSE9309 |
Recovery of biological information under heterogeneous experimental conditions using subgroup standardization. |
| GSE32641 |
Major functional transcriptome of an inferred center regulator of an ER(-) breast cancer model system |
|
| Relations |
| Reanalyzed by |
GSE24124 |
