|
| Status |
Public on Nov 04, 2016 |
| Title |
MG1363_Hexanol_5min_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MG1363, Hexanol (2 mg/ml), 5 min, Biological Replica 1
|
| Organism |
Lactococcus cremoris subsp. cremoris MG1363 |
| Characteristics |
treatment: Hexanol (2 mg/ml), 5 min
|
| Treatment protocol |
Growth was stopped immidiately by transfer of 10 ml culture sample into a glass flask precolled on ice followed rapid swirling
|
| Growth protocol |
Exponentially growing strains in SA medium with 1% glucose and 2 ug/ml lipoic acid. Alcohol stress (Ethanol 55 mg/ml, butanol 10mg/ml, or hexanol 2 mg/ml) were added following harvesting the references and the stressed cultures were harvested after 5 min and 60 min
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were pelleted by centrifugation and washed in ice cold salin water (0.9% NaCl). RNA was extracted by the hot phenol method
|
| Label |
Cy3
|
| Label protocol |
Labbeling according to aglient protocol using random hexamer and Cy3
|
| |
|
| Hybridization protocol |
Hydridzation according to Aglient Protocol. Aglient 8x15k custom microarray G2509F
|
| Scan protocol |
Aglient technologies scanner G2505C. Aglient’s Feature Extraction Software
|
| Data processing |
Quantile normalization
|
| |
|
| Submission date |
Nov 03, 2016 |
| Last update date |
Nov 04, 2016 |
| Contact name |
Mogens Kilstrup |
| E-mail(s) |
mki@bio.dtu.dk
|
| Phone |
+45 25538815
|
| Organization name |
Technical University of Denmark
|
| Department |
DTU Systems Biology
|
| Lab |
Metabolic Signaling and Regulation
|
| Street address |
Building 301 room 158
|
| City |
Kgs. Lyngby |
| ZIP/Postal code |
DK2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL21561 |
| Series (1) |
| GSE89508 |
Gene expression signature in Lactococcus lactis after ethanol, butanol or hexanol exposure |
|
