|
| Status |
Public on Nov 01, 2007 |
| Title |
rhPBEF & VALI rep1 (age 8-12 weeks) |
| Sample type |
RNA |
| |
|
| Source name |
Lung
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6J
22-32g
Age: 8-12 week
|
| Biomaterial provider |
Charles Rivers Laboratories (Wilmington, MA)
|
| Treatment protocol |
rhPBEF 20µg / mouse in 1.5µl water per gram body weight, intratracheal injection; VILI (4 hours, 30 ml/kg tidal volume)
|
| Growth protocol |
standard conditions (12h light-dark cycle, 25-27ºC, ~40% humidity)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse320_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from lung tissue of control animal
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) for ABS_Call; Bioconductor GCRMA package for normalization;
|
| |
|
| Submission date |
Oct 18, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yong Huang |
| E-mail(s) |
yh9fj@virginia.edu
|
| Phone |
(434) 243-0842
|
| Organization name |
University of Viginia
|
| Department |
Medicine
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9368 |
Mouse lung with recombinant human soluble PBEFtreatment and ventilator-induced lung injury: age 8-12wks |
|
| Relations |
| Reanalyzed by |
GSE119085 |
