|
| Status |
Public on Mar 13, 2017 |
| Title |
Fall'13 Day 42 Control Illinois Heart Fat 1110 |
| Sample type |
SRA |
| |
|
| Source name |
Cardiac Adipose Tissue
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Cardiac Adipose
breed/line: Illinois
gender: male
age of collection: Day 42 posthatch
|
| Treatment protocol |
First week house temperature: 35 degrees C with 5 degrees C decrease each week until 25 degrees celsius was reached. The house maintained this temperature through completion of the trial at day 42 (typical market age).
|
| Growth protocol |
Birds were raised following grower standards outlined by our AACUC protocol (#(27)03-12-14R). Briefly, birds were raised on litter with ad libitum access to feed and water. First week house temperature: 35 degrees C with 5 degrees C decrease each week until 25 degrees celsius was reached. The house was maintained this temperature through completion of the trial at day 42 (typical market age). The house received 23 hours of light and 1 hour of dark for the entirety of the study. Necropsies were performed on Day 21, Day 22, Day 26, Day 32 and Day 42.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Birds were euthanized via cervical dislocation and necropsied on each day listed above. Abdominal adipose tissue and cardiac adipose tissue samples were collected and flash frozen with liquid nitrogen.
Libraries were constructed using the Illumina Stranded RNA-seq kit according to manufacturers instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Bowtie
TopHat
Cufflinks
Genome_build: Gallus gallus ver 4
|
| |
|
| Submission date |
Nov 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Carl Schmidt |
| E-mail(s) |
cjschmidt79@gmail.com
|
| Organization name |
University of Delaware
|
| Street address |
601 Sincock Ln
|
| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19716 |
| Country |
USA |
| |
|
| Platform ID |
GPL19005 |
| Series (1) |
| GSE89643 |
Exploration of the Chicken Adipose Tissue |
|
| Relations |
| BioSample |
SAMN06565743 |
| SRA |
SRX2637437 |
