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Series GSE89643 Query DataSets for GSE89643
Status Public on Mar 13, 2017
Title Exploration of the Chicken Adipose Tissue
Organism Gallus gallus
Experiment type Expression profiling by high throughput sequencing
Summary Adipose tissue is essential for lipid storage and thus, energy regulation and maintenance. Many organs in the body utilize lipids as their primary energy source, such as the heart. In addition, when glucose supplies are insufficient adipose tissue is broken down and fats are utilized systemically as a primary energy source. This is regulated by the endocrine system primarily through the regulation of glucagon and insulin. Adipose tissue is also involved with the immune system particularly in eliciting an inflammatory response through cytokine signaling and the release of interleukins. Chickens have two major fat pads: an abdominal fat pad and a cardiac fat pad. The abdominal fat pad is located on the ventral side, above the GI tract. The cardiac fat pad is situated on the atria of the heart and surrounds the aorta. These fat pads are different in coloration and size which leads to the hypothesis that these fat pads have different functions. The objectives of the project are to (a) identify genes that are unique to each type of adipose tissue when compared to the rest of the body, through the collection of other tissues and (b) to identify genes that are differentially expressed in each type of adipose tissue in comparison to each other to better understand the functioning of these fat pads in the chicken and the pathways that they are involved in at the transcriptomic level.
 
Overall design Male Ross 708 broiler chickens were raised from day of hatch to day 42, typical market age, on the University of Delaware farm. Birds were placed into a house. The house was kept at 23 hours of light and 1 hour of dark and birds were placed on litter and given feed (meeting all NRC requirements) and water with ad libitum access. The house was kept at 35 degrees celsius for the first week and the temperature was decreased 5 degrees celsius each week until 25 degrees celsius. The house was maintained at 25 degrees celsius for the remainder of the study. Necropsies were performed at several points throughout the trial (days 21, 22, 26, 32, and 42).
 
Contributor(s) Schmidt CJ, Schneider BK
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Submission date Nov 08, 2016
Last update date May 15, 2019
Contact name Carl Schmidt
E-mail(s) cjschmidt79@gmail.com
Organization name University of Delaware
Street address 601 Sincock Ln
City Newark
State/province DE
ZIP/Postal code 19716
Country USA
 
Platforms (1)
GPL19005 Illumina HiSeq 2500 (Gallus gallus)
Samples (31)
GSM2385895 Fall'13 Day 42 Control Illinois Ab Fat 1092
GSM2385896 Fall'13 Day 42 Control Illinois Ab Fat 1093
GSM2385897 Fall'13 Day 42 Control Illinois Ab Fat 1094
Relations
BioProject PRJNA379019
SRA SRP101814

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89643_Chicken_Adipose_gene_expression.tsv.gz 71.7 Kb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record
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