|
| Status |
Public on Nov 18, 2016 |
| Title |
15 minutes before ndt80 block release_timecourse_II |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
A14201_15 minutes before ndt80 block release
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: A14201
timepoint: 15 minutes before ndt80 block release
|
| Treatment protocol |
After 6 hours in sporulation medium, 1μM beta-estradiol is added to the culture to induce NDT80 expression.
|
| Growth protocol |
yeast cells were grow in YPD plate overnight, in YPD liquid medium to saturation, diluted in YPA medium and cultured overnight, shift to sporulation medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted by Ribopure yeast kit (Ambion) (Am1926)
|
| Label |
Cy3
|
| Label protocol |
Agilent Quick Amp labeling kit two color (5190-0441)
|
| |
|
| Channel 2 |
| Source name |
A14201 in Ndt80 block (6 hours in SPO medium)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: A14201
|
| Treatment protocol |
After 6 hours in sporulation medium, 1μM beta-estradiol is added to the culture to induce NDT80 expression.
|
| Growth protocol |
yeast cells were grow in YPD plate overnight, in YPD liquid medium to saturation, diluted in YPA medium and cultured overnight, shift to sporulation medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted by Ribopure yeast kit (Ambion) (Am1926)
|
| Label |
Cy5
|
| Label protocol |
Agilent Quick Amp labeling kit two color (5190-0441)
|
| |
|
| |
| Hybridization protocol |
Agilent gene expression Hybridization kit (5188-5242)
|
| Scan protocol |
Agilent G2505B serial # US451R2860 pixel 5
|
| Data processing |
Agilent Feature Extraction 9.501
|
| |
|
| Submission date |
Nov 17, 2016 |
| Last update date |
Nov 18, 2016 |
| Contact name |
Bruce Futcher |
| Organization name |
Stony Brook University
|
| Department |
Molecular Genetics & Microbiology
|
| Lab |
Life Sciences Building Rm363
|
| Street address |
STONY BROOK UNIVERSITY
|
| City |
Stony Brook |
| State/province |
NY |
| ZIP/Postal code |
11794-5222 |
| Country |
USA |
| |
|
| Platform ID |
GPL9294 |
| Series (2) |
| GSE90006 |
Yeast (Saccharomyces cerevisiae) synchronized meiotic timecourse II |
| GSE90009 |
Synchronized yeast meiotic culture timecourse |
|
