|
| Status |
Public on Nov 07, 2007 |
| Title |
Testes, 20 hours after cadmium treatment, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
20 hour after cadmium treatment
|
| Organism |
Rattus norvegicus |
| Characteristics |
medium to late response after cadmium treatment
|
| Treatment protocol |
3 mg/kg b.w. cadmium chloride injected via i.p.
|
| Growth protocol |
Adult male SD rats
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by Qiagen's Rneasy kit purification
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
gene expression data from rat testis 20 h after cadmium treatment
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
|
| |
|
| Submission date |
Oct 31, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
C YAN CHENG |
| E-mail(s) |
yan@popcbr.rockefeller.edu
|
| Phone |
212-327-8738
|
| Fax |
212-327-8733
|
| Organization name |
POPULATION COUNCIL
|
| Street address |
1230 YORK AVENUE
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE9480 |
Expression data from adult rat testes treated with 3 mg/kg b.w. cadmium chloride via i.p. |
|
