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Sample GSM241888

Query DataSets for GSM241888

Status

Public on Nov 13, 2007

Title

pRb-/-;p107-/-1

Sample type

RNA

Source name

Whole skin tissue

Organism

Mus musculus

Characteristics

Rb F19/F19; p107-/-; K14 cre
age: newborn
strain: FVB
tissue: skin

Extracted molecule

total RNA

Extraction protocol

Mouse skin tissue, obtained after birth, was preserved in RNAlater (Ambion), and disrupted and homogenized using Mixer Mill MM301 (Retsch). Total RNA was extracted and purified from 30 mg of skin using RNeasy Fibrous Tissue Mini kit (Qiagen) following manufacturers' recommendations

Label

Biotin

Label protocol

Affymetrix protocol

Hybridization protocol

Affymetrix protocol

Scan protocol

Affymetrix protocol

Description

RNA integrity tested with Bioanalyzer (Agilent)

Data processing

RMA normalization, log2 transformed, probe value mean-centered, mean=0, stdv=1

Submission date

Nov 08, 2007

Last update date

Aug 14, 2011

Contact name

Ramon Garcia-Escudero

E-mail(s)

ramon.garcia@ciemat.es, rgescudero@gmail.com

Organization name

CIEMAT

Department

Biomedical Innovation

Lab

Molecular and Traslational Oncology

Street address

Avda Complutense 40, Ed 70A

City

Madrid

State/province

Madrid

ZIP/Postal code

28040

Country

Spain

Platform ID

GPL339

Series (1)

GSE9567

p107 acts as a tumor suppressor in pRb-deficient epidermis.

Data table header descriptions
ID_REF
VALUE	log2 signal values, probes mean centered, mean=0 and stdv=1

Data table
ID_REF	VALUE
1415670_at	-1.1308858
1415671_at	-0.8382869
1415672_at	-0.45246714
1415673_at	-0.51651776
1415674_a_at	-1.7578107
1415675_at	-0.7618006
1415676_a_at	-0.9227662
1415677_at	0.3432108
1415678_at	1.4239837
1415679_at	0.39533794
1415680_at	0.34817442
1415681_at	-0.47415093
1415682_at	0.45754343
1415683_at	-0.70818776
1415684_at	-1.0990803
1415685_at	-0.8478781
1415686_at	0.17974564
1415687_a_at	1.266886
1415688_at	0.2959253
1415689_s_at	1.3828899

Total number of rows: 22690

Table truncated, full table size 501 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM241888.CEL.gz	3.4 Mb	(ftp)(http)	CEL
Processed data included within Sample table