|
| Status |
Public on Jan 01, 2018 |
| Title |
Control 1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary effusion lymphoma cell-line BCP1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BCP1
agent: vehicle control
|
| Treatment protocol |
The cells were treated with vehicle control or RR inhibitor 3-AP (2.5 µM) for 48 h
|
| Growth protocol |
The cells were cultured in RPMI 1640 medium (ATCC) supplemented with 20% FBS. All cells were cultured at 37°C in 5% CO2. All experiments were carried out using cells harvested at low (< 20) passages.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TriZol
|
| Label |
Cy3
|
| Label protocol |
cRNA was labeled with the TargetAmp-Nano Labeling kit for Illumina Expression BeadChip (Epicentre),
|
| |
|
| Hybridization protocol |
Hybridization using standard hybridization protocols from Illumina for 14h at 58°C
|
| Scan protocol |
Standard Illumina protocols for HumanHT-12 V4 chips from Illumina using the Illumina iScanner
|
| Description |
Sample1
|
| Data processing |
Background signal was removed using the Detection P value algorithm. Signals were normalized using the cubic spline algorithm. Differential expression was determined using the Illumina Custom Algorithm. Ratios were determined as the fraction between the treatments and the Control group
|
| |
|
| Submission date |
Dec 09, 2016 |
| Last update date |
Jan 01, 2018 |
| Contact name |
Zhiqiang Qin |
| E-mail(s) |
zqin@lsuhsc.edu
|
| Organization name |
LSUHSC-NO
|
| Street address |
1700 Tulane Ave
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE91389 |
Targeting ribonucleotide reductase by 3-AP in primary effusion lymphoma |
|
