|
| Status |
Public on Nov 15, 2007 |
| Title |
Monocyte_HCMV-1 |
| Sample type |
RNA |
| |
|
| Source name |
HCMV-infected monocytes at 4 hours post infection
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: Peripheral blood
Virus Strain: Towne/E
|
| Treatment protocol |
Isolated monocytes were pretreated nonadherently at 37C for 45 minutes with 5 μM of the NF-κB inhibitor Bay11-7082, 50 μM of the PI(3)K inhibitor LY294002, or dimethyl sulfoxide (DMSO) as the solvent control prior to infection. Cells were then HCMV infected (MOI 15) and incubated nonadherently at 37C for 4 hours.
|
| Growth protocol |
Monocytes were incubated in RPMI supplemented with 10% human AB serum at 37C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA STAT-60 extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95Av2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
HCMV-infected monocyte, biological rep1
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling with a target intensity value of 2500 as normalization method.
|
| |
|
| Submission date |
Nov 14, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Andrew D. Yurochko |
| E-mail(s) |
ayuroc@lsuhsc.edu
|
| Phone |
318-675-8332
|
| Fax |
318-675-5764
|
| Organization name |
LSUHSC-S
|
| Department |
Microbiology & Immunology
|
| Street address |
1501 Kings Highway
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71130-3932 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (2) |
| GSE9601 |
Expression Data From HCMV-Infected Human Monocytes |
| GSE11408 |
Expression Data From HCMV-Infected Human Monocytes Study 2 |
|
