|
| Status |
Public on May 30, 2008 |
| Title |
B6 WT_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Cornea
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C.129S2(B6)-Il8rbtm1Mwm/J (Il8rb-/-)
Age: 14 day
Gender: Mixed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 20 corneas (10 mice) with Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA) and purified with the Rneasy Mini Kit Qiagen, Valencia, CA).
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed by using 5.5 µg of total RNA according to the manufactures instructions as described in the GeneChip Expression Analysis Technical Manual (Affymetrix, 2004) with Affymetrix IVT amplification and labeling kit.
|
| |
|
| Hybridization protocol |
10 µg of purified, fragmented labeled cRNA was hybridized according to the manufactures instructions as described in the GeneChip Expression Analysis Technical Manual (Affymetrix, 2004) in Affymetrix 640 hybridization oven.
|
| Scan protocol |
Scanning was performed according to the manufactures instructions as described in the GeneChip Expression Analysis Technical Manual (Affymetrix, 2004) on Affymetrix GC3000 G7 scanner.
|
| Description |
Gene expression data from corneas of 14 day old of C.129S2(B6)-Il8rbtm1Mwm/J (Il8rb-/-)(B6 wild type)
|
| Data processing |
The data was generated from normalizing the raw data by using GCRMA Version 2.0.0 by using the R statistic 2.4.1.
|
| |
|
| Submission date |
Nov 30, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Sakae Ikeda |
| E-mail(s) |
ikeda@wisc.edu
|
| Phone |
608-262-5991
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Department of Medical Genetics
|
| Street address |
425-G Henry Mall Room 5350 Genetics/Biotech
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9743 |
The in vivo effect of actin cytoskeletal abnormalities on gene expression |
|
| Relations |
| Reanalyzed by |
GSE119085 |
