|
| Status |
Public on Dec 04, 2007 |
| Title |
Exp A_1, 50mg/kg CPF |
| Sample type |
RNA |
| |
|
| Source name |
Brain tissue from Fisher 344 male rat treated with 50mg/kg CPF
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sex: male
Age: adult (11-12 weeks)
|
| Treatment protocol |
F344 male rats were treated with a single dose (0, 0.5, 1.0, 5.0, 10, 30, or 50mg/kg) of CPF (Sigma) in three separate experiments and sacrificed after 96 hours of exposure to CPF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen MiniRNeasy total RNA extraction was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the RNA transcript labeling procedures of Enzo from 15ug of total RNA
|
| |
|
| Hybridization protocol |
After fragmentation 15ug of cRNA was hybridized for 16 hours @ 45C on GeneChip Rat Expression Array 230A. GeneChips were washed and stained in an Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Description |
Exp A, Animal #1, 50mg/kg CPF
Gene expression data from male F344 rats treated with a single dose of 50mg/kg of CPF for 96 hours
|
| Data processing |
Data was analyzed with Data Mining Tool version 3.0 (DMT 3.0) using the following parameters: Alpha1=0.05; Alpha2=0.06; Tau=0.015; Gamma1H=0.005; Gamma1L=0.005; Gamma2H=0.006; Gamma2L=0.006; Perturbation=1.35; Scaling (All Probe Sets) Target Intensity=500
|
| |
|
| Submission date |
Dec 03, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Victor Chan |
| E-mail(s) |
victor.chan@wpafb.af.mil, andrea.stapleton@wpafb.af.mil
|
| Phone |
9379049501
|
| Organization name |
Air Force Research Laboratory
|
| Department |
Applied Biotechnology Branch
|
| Street address |
2729 R St. Area B Bldg. 837
|
| City |
WPAFB |
| State/province |
OH |
| ZIP/Postal code |
45433-5707 |
| Country |
USA |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE9751 |
Expression data from brain tissue of Rattus norvegicus treated with chlorpyrifos (CPF) |
|
