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Sample GSM2461440 Query DataSets for GSM2461440
Status Public on Apr 19, 2017
Title Liver_8W-BS-rep2
Sample type SRA
Source name 8-week-old Mouse liver
Organism Mus musculus
Characteristics strain: C57BJ/6
gender: male
age: 8 week
tissue: liver
Extracted molecule total RNA
Extraction protocol mRNAs of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement.
Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA)
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2500
Description bisulfite treated mRNA
8-week-old Mouse liver
Data processing Raw Reads were stripped of adaptor sequence and removed low quality bases using Trimmomatic, clean reads were aligned to the hg19 or mm10 genome assembly using bismark
The m5C sites were called by bismark.
Genome_build: hg19 or mm10
Supplementary_files_format_and_content: Human_m5C_sites_information.xls, m5C_sites_in_ALYREF-RIP.xls, Mouse_m5C_sites_information.xls
Submission date Jan 18, 2017
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL17021
Series (2)
GSE93749 Single-base resolution methylome of 5-Methylcytosine in human and mouse transcriptome
GSE93751 5-methylcytosine promotes mRNA export
BioSample SAMN06237519
SRA SRX2499536

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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