|
| Status |
Public on Jan 20, 2017 |
| Title |
P.gingivalis W83 wild strain rep2 |
| Sample type |
RNA |
| |
|
| Source name |
P.gingivalis W83 wild strain, replicate 2
|
| Organism |
Porphyromonas gingivalis |
| Characteristics |
strain: W83
treatment: none
|
| Treatment protocol |
Porphyromonas gingivalis strain W83 was treated with 6.25ug/mL for 0.5h.
|
| Growth protocol |
Porphyromonas gingivalis strain W83 was grown anaerobically (10% CO2, 10% H2, 80% N2 ) in enriched brain–heart infusion (BHI) broth containing 5% fiber-free sheep blood, 1% vitamin K and hemin, 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent and was further purified using NucleoSpin® RNA clean-up（MACHEREY-NAGEL，Germany） according to the manufactures’ instructions. The RNA quality was assessed by formaldehyde agarose gel electrophoresis and was quantitated spectrophotometrically.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed at the CapitalBio Corporation (http://www.capitalbio.com/en/index.asp, Beijing, China), a service provider authorized by Agilent (California, USA) according to the Agilent’s Expression user’s guide.
|
| |
|
| Hybridization protocol |
Hybridization was performed at the CapitalBio Corporation (http://www.capitalbio.com/en/index.asp, Beijing, China), a service provider authorized by Agilent (California, USA), according to the Agilen’s Expression user’s guide.
|
| Scan protocol |
Scanning was performed at the CapitalBio Corporation (http://www.capitalbio.com/en/index.asp, Beijing, China), a service provider authorized by Agilent (California, USA), according to the Agilen’s Expression user’s guide.
|
| Description |
Gene expression of P. gingivalis W83 wild strain
|
| Data processing |
Data analysis was performed at the CapitalBio Corporation (http://www.capitalbio.com/en/index.asp, Beijing, China), a service provider authorized by Agilent (California, USA) . The expression data of probes were normalized using quantile normalization and expression data of genes were generated using the Robust Multichip Average (RMA) algorithm.
|
| |
|
| Submission date |
Jan 19, 2017 |
| Last update date |
Jan 20, 2017 |
| Contact name |
Hongyan Wang |
| E-mail(s) |
hywang@cmu.edu.cn
|
| Organization name |
School of Stomatology, China Medical University
|
| Department |
Department of Periodontology
|
| Street address |
Nanjing North St. 117
|
| City |
Shenyang |
| State/province |
Liaoning Province |
| ZIP/Postal code |
110002 |
| Country |
China |
| |
|
| Platform ID |
GPL22953 |
| Series (1) |
| GSE93873 |
Molecular pathways underlying inhibitory effect of antimicrobial peptide Nal-P-113 on bacteria biofilms formation of Porphyromonas gingivalis W83 by DNA microarray |
|
