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Status |
Public on Mar 20, 2008 |
Title |
7-day-old, biological rep1 |
Sample type |
RNA |
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|
Source name |
non-infected mouse intestinal tissue
|
Organism |
Mus musculus |
Characteristics |
BALB/c Mice Age: 7 day-old mice
|
Treatment protocol |
After 2 or 4 hours of intra-gastric infection with Shigella, a section of intestin was removed and extracted.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, following by clean-up ising RNEasy Mini kit from Qiagen.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix MOE430A probe array cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
After staining using PE-streptavidin, arrays were scanned at 488 nm using an Argon-ion laser
|
Description |
RNA from intestinal tissue of 4-day or 7-day mice infected or not with invasive or non-invasive Shigella strains for 2 or 4 hours
|
Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
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|
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Submission date |
Dec 05, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Thierry Pedron |
E-mail(s) |
tpedron@pasteur.fr
|
Phone |
33 1 40 61 36 11
|
Fax |
33 1 45 68 89 53
|
Organization name |
Pasteur Institute
|
Department |
Biologie cellulaire et Infection
|
Lab |
Pathogénie Microbienne Moléculaire
|
Street address |
28 rue du Docteur Roux
|
City |
Paris |
ZIP/Postal code |
75015 |
Country |
France |
|
|
Platform ID |
GPL339 |
Series (1) |
GSE9785 |
Expression data from Newborn mice infected with Shigella flexneri |
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