|
| Status |
Public on Mar 20, 2008 |
| Title |
4-day-old, infected during 4 hours with INV+,biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
infected with invasive (INV+) mouse intestinal tissue
|
| Organism |
Mus musculus |
| Characteristics |
BALB/c Mice
Age: 4 day-old mice
|
| Treatment protocol |
After 2 or 4 hours of intra-gastric infection with Shigella, a section of intestin was removed and extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, following by clean-up ising RNEasy Mini kit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix MOE430A probe array cartridge. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
After staining using PE-streptavidin, arrays were scanned at 488 nm using an Argon-ion laser
|
| Description |
RNA from intestinal tissue of 4-day or 7-day mice infected or not with invasive or non-invasive Shigella strains for 2 or 4 hours
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 05, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Thierry Pedron |
| E-mail(s) |
tpedron@pasteur.fr
|
| Phone |
33 1 40 61 36 11
|
| Fax |
33 1 45 68 89 53
|
| Organization name |
Pasteur Institute
|
| Department |
Biologie cellulaire et Infection
|
| Lab |
Pathogénie Microbienne Moléculaire
|
| Street address |
28 rue du Docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE9785 |
Expression data from Newborn mice infected with Shigella flexneri |
|
