|
| Status |
Public on Jul 31, 2017 |
| Title |
cyp20-3 at normal light, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
cyp20-3 at normal light_shoot
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: CYP20-3
sample group: Control
tissue: shoot
|
| Treatment protocol |
Plants were grown under normal light conditions (80 µmol quanta m-2s-1) or treated with 6 h high light (800 µmol quanta m-2s-1)
|
| Growth protocol |
Arabidopsis thaliana wildtype and mutants were grown in soil for total 4 weeks under short-day conditions (8 h light / 16 h dark)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the peqGOLD Total RNA Kit according to the manufacturer´s instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
C_CYP20_3_3
Gene expression data from shoots of 28-day-old Arabidopsis cyp20-3 grown in normal light
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of Aragene_1.0st-type from Affymetrix. A Custom CDF Version 20 with TAIR based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Jan 31, 2017 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Carolina Delatorre |
| E-mail(s) |
carolina.delatorre@medma.uni-heidelberg.de
|
| Organization name |
University Heidelberg
|
| Street address |
Theodor-Kutzer-Ufer 1-3
|
| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23011 |
| Series (1) |
| GSE94327 |
The redox-sensitive regulatory module of cyclophilin 20-3, 2-cysteine peroxiredoxin and cysteine synthase in the high light acclimation response in Arabidopsis thaliana |
|
