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Sample GSM2473208 Query DataSets for GSM2473208
Status Public on Jul 31, 2017
Title oastlB at 6 h high light, biological replicate 3
Sample type RNA
 
Source name oastlB at 6 h high light_shoot
Organism Arabidopsis thaliana
Characteristics genotype: oastlB
sample group: 6 h high light
tissue: shoot
Treatment protocol Plants were grown under normal light conditions (80 µmol quanta m-2s-1) or treated with 6 h high light (800 µmol quanta m-2s-1)
Growth protocol Arabidopsis thaliana wildtype and mutants were grown in soil for total 4 weeks under short-day conditions (8 h light / 16 h dark)
Extracted molecule total RNA
Extraction protocol RNA was extracted using the peqGOLD Total RNA Kit according to the manufacturer´s instruction
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description S_oastlB_3
Gene expression data from shoots of 28-day-old Arabidopsis oastlB grown in 6 h high light
Data processing The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of Aragene_1.0st-type from Affymetrix. A Custom CDF Version 20 with TAIR based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
 
Submission date Jan 31, 2017
Last update date Jul 31, 2017
Contact name Carolina Delatorre
E-mail(s) carolina.delatorre@medma.uni-heidelberg.de
Organization name University Heidelberg
Street address Theodor-Kutzer-Ufer 1-3
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platform ID GPL23011
Series (1)
GSE94327 The redox-sensitive regulatory module of cyclophilin 20-3, 2-cysteine peroxiredoxin and cysteine synthase in the high light acclimation response in Arabidopsis thaliana

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AT1G01010_at 5.728515625
AT1G01020_at 5.854492188
AT1G01030_at 2.510253906
AT1G01040_at 6.494140625
AT1G01046_at 2.8828125
AT1G01050_at 7.236328125
AT1G01060_at 3.277832031
AT1G01070_at 1.940917969
AT1G01073_at 1.231933594
AT1G01080_at 9.03515625
AT1G01090_at 9.1328125
AT1G01100_at 8.103515625
AT1G01110_at 4.778320313
AT1G01115_at 2.323242188
AT1G01120_at 7.213867188
AT1G01130_at 3.265136719
AT1G01140_at 8.482421875
AT1G01150_at 2.61328125
AT1G01160_at 7.618164063
AT1G01170_at 8.80078125

Total number of rows: 27826

Table truncated, full table size 667 Kbytes.




Supplementary file Size Download File type/resource
GSM2473208_Wirtz_Shanshan_290616_oastlB-S3_AraGene-1_0-st_.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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