|
| Status |
Public on Sep 25, 2017 |
| Title |
Tum008 |
| Sample type |
RNA |
| |
|
| Source name |
primary tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: N-Myc NCC-derived neuroblastoma
|
| Growth protocol |
N-Myc transduced cells are a mixture of both p53+/- and p53-/- NCCs and are designated as N-Myc; p53mixed. At 48 hours post infection, N-Myc; p53mixed NCCs were harvested and ~10,000 cells were injected subcutaneously into nude mice or into C57Bl/6 mice After ~ 50 days primary tumors were removed from the mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted with Trizol reagent (Invitrogen) according to a supplied RNA isolation protocol.
|
| Label |
Biotin (Affyexpress)
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Followed standard protocol for Affymetrix Mouse 2.0 ST arrays.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
Neuroblastoma tumor model
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.5 using the RMA method
|
| |
|
| Submission date |
Feb 15, 2017 |
| Last update date |
Sep 25, 2017 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE94914 |
MYCN induces neuroblastoma in primary neural crest cells |
|
