|
| Status |
Public on Jun 23, 2017 |
| Title |
DNA methylation in NT cells_ Replicate 2 |
| Sample type |
genomic |
| |
|
| Source name |
Cervical carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
cell type: cervical carcinoma cell line
treatment: nontreated
|
| Treatment protocol |
iAsT cells were treated with 0.5 uM Sodium Arsenite for 36 days; iAsT-rev cells were treated with 0.5 uM Sodium Arsenite for 36 days and were then treated with dI water, cells were harvested 10 days after reversal of treatment.
|
| Growth protocol |
Cell cultures were incubated at 37 degree with humidified air and 5% CO2. Cells were harvested after each treatment. Cells were grown ensuring that 95% of the cells were viable. Harvested cells were centrifuged for five minutes at 200 X g.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Qiagen DNeasy kit according to manufacturer's instructions.
|
| Label |
unknown
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulphite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation EPIC BeadChip using standard Illumina EPIC protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina HiScan.
|
| Data processing |
Minfi preprocessFunNorm.
|
| |
|
| Submission date |
Feb 22, 2017 |
| Last update date |
May 28, 2018 |
| Contact name |
Yvonne Nsokika Fondufe-Mittendorf |
| E-mail(s) |
y.fondufe-mittendorf@uky.edu
|
| Phone |
8593230091
|
| Organization name |
University of Kentucky
|
| Department |
Biochemistry
|
| Lab |
Mittendorf
|
| Street address |
741 South Limestone dr.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536-0509 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE95232 |
Transient and permanent changes in DNA methylation patterns in inorganic arsenic-mediated epithelial-to-mesenchymal transition |
|
