|
| Status |
Public on Mar 09, 2017 |
| Title |
m_SET33_CTL_(MoGene-2_0-st) |
| Sample type |
RNA |
| |
|
| Source name |
3T3-L1_cocultured with_none (control)
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 3T3-L1
cell type: adipocyte cell line
cocultured with: none (control)
|
| Treatment protocol |
3T3-L1 cells were cultured in a indirect co-culture wells with breast cancer cells
|
| Growth protocol |
3T3-L1 cells were cultured in DMEM supplemented with 10% bovine serum at 37°C in 5% CO2. 3T3-L1 cells were grown to confluency and induced with adipogenesis media (DMEM with 10% FBS, 10μg/ml insulin, 0.5mM IBMX and 1μM dexamethasone). After 2 days, the media was changed with DMEM with 10% FBS and 10μg/ml insulin and maintained for 4 days. After 8 days of differentiation with DMEM with 10% FBS, the cells were used for further experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit.
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Human 2.0 ST Array at 45°C for 16hour.
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Description |
in-direct co-culture method
|
| Data processing |
Raw data were extracted automatically in Affymetrix data extraction protocol using the software provided by Affymetrix GeneChip® Command Console® Software (AGCC). After importing CEL files, the data were summarized and normalized with robust multi-average (RMA) method implemented in Affymetrix® Expression Console™ Software (EC).
|
| |
|
| Submission date |
Mar 08, 2017 |
| Last update date |
Mar 09, 2017 |
| Contact name |
Hyeong-Gon Moon |
| E-mail(s) |
moonhgsurgi@gmail.com
|
| Organization name |
Seoul National University Hospital
|
| Department |
Surgery
|
| Street address |
101, Daehak-ro Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
03080 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE95827 |
Cancer-associated adipocytes mRNA |
| GSE95829 |
Transition into inflammatory cancer-associated adipocytes in breast cancer microenvironment requires microRNA regulatory mechanism |
|
