|
Status |
Public on Jun 17, 2008 |
Title |
small airways, COPD 080 |
Sample type |
RNA |
|
|
Source name |
airway epithelial cells obtained by bronchoscopy and brushing
|
Organism |
Homo sapiens |
Characteristics |
age: 46 sex: M ethnic group: hispanic smoking status: COPD, GOLD-I, 22 pack-years
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
Description |
Comparison of gene expression in airway epithelial cells of normal non-smokers, phenotypic normal smokers, smokers with early COPD, and smokers with COPD.
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Dec 21, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Yael Strulovici-Barel |
E-mail(s) |
yas2003@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
Department of Genetic Medicine
|
Lab |
Crystal
|
Street address |
1300 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (7)
|
GSE8545 |
Variability in Small Airway Epithelial Gene Expression Among Normal Smokers |
GSE10006 |
Decreased Expression of Intelectin 1 in The Human Airway Epithelium of Smokers Compared to Nonsmokers |
GSE11784 |
Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking |
GSE11906 |
Quality Control in Microarray Assessment of Gene Expression in Human Airway Epithelium |
GSE19407 |
Smoking-induced Wnt pathway downregulation |
GSE20257 |
Smoking-induced Disarray of the Apical Junctional Complex Gene Expression Architecture in the Human Airway Epithelium |
GSE21359 |
Association of CXCL14 in the Human Airway Epithelium with Chronic Obstructive Lung Disease and Lung Cancer |
|
Relations |
Reanalyzed by |
GSE60486 |
Reanalyzed by |
GSE119087 |