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| Status |
Public on Dec 19, 2008 |
| Title |
Decreased Expression of Intelectin 1 in The Human Airway Epithelium of Smokers Compared to Nonsmokers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Lectins are proteins present on cell surfaces or as shed extracellular proteins that function in innate immune defense as phagocytic receptors to recognize specific bacterial cell wall components. Based on the knowledge that cigarette smoking is associated with increased risk of bacterial infection, we hypothesized that cigarette smoking may modulate the expression of lectin genes in the airway epithelium. Affymetrix HG U133 Plus 2.0 microarrays were used to survey expression of lectin genes in large (3rd to 4th order bronchi) airway epithelium from 9 normal nonsmokers and 20 phenotypic normal smokers and small (10th to 12th order bronchi) airway epithelium from 13 normal nonsmokers and 20 phenotypic normal smokers. From the 72 lectin genes that were surveyed, there were no changes (>2-fold change, p<0.05) in gene expression in either large or small airway epithelium among normal smokers compared to nonsmokers except for a striking down regulation in both large and small airway epithelium of normal smokers of intelectin 1, a recently described lectin that participates in the innate immune response by recognizing and binding to galactofuranosyl residues in the cell walls of bacteria (large airway epithelium, p<0.003; small airway epithelium, p<0.002). TaqMan RT-PCR confirmed the observation that intelectin 1 was down-regulated in both large (p<0.05) and small airway epithelium (p<0.02) of normal smokers compared to normal nonsmokers. Immunohistochemistry assessment of biopsies of the large airway epithelium of normal nonsmokers demonstrated intelectin 1 was expressed in secretory cells, with qualitatively decreased expression in biopsies from normal smokers. Western analysis confirmed the decreased expression of intelectin 1 in airway epithelium of normal smokers compared to normal nonsmokers (p<0.02). Finally, compared to normal nonsmokers, intelectin 1 expression was decreased in small airway epithelium of smokers with early COPD (n= 13, p<0.001) and smokers with established COPD (n= 14, p<0.001), in a fashion similar to that of normal smokers. In the context that intelectin 1 is an epithelial molecule that likely plays a role in defense against bacteria, the down regulation of expression of intelectin 1 in response to cigarette smoking may contribute to the increase in susceptibility to infections observed in smokers, including those with COPD.
Keywords: COPD
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| Overall design |
Comparison of gene expression in airway epithelial cells of normal non-smokers, phenotypic normal smokers, smokers with early COPD, and smokers with COPD.
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| Contributor(s) |
Carolan B, Harvey B, De BP, Vanni H, Crystal RG |
| Citation(s) |
18832735 |
| |
| Submission date |
Dec 21, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
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| Lab |
Crystal
|
| Street address |
1300 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (87)
|
| GSM252802 |
large airways, non-smoker 008 |
| GSM252803 |
large airways, non-smoker 011 |
| GSM252804 |
large airways, non-smoker 030 |
| GSM252805 |
large airways, non-smoker 035 |
| GSM252806 |
large airways, non-smoker 042 |
| GSM252807 |
large airways, non-smoker 044 |
| GSM252808 |
large airways, smoker 002 |
| GSM252809 |
large airways, smoker 003 |
| GSM252810 |
large airways, smoker 023 |
| GSM252811 |
large airways, smoker 025 |
| GSM252812 |
large airways, smoker 049 |
| GSM252813 |
large airways, smoker 057 |
| GSM252814 |
large airways, smoker 058 |
| GSM252815 |
large airways, smoker 063 |
| GSM252816 |
large airways, smoker 069 |
| GSM252817 |
large airways, smoker 074 |
| GSM252818 |
large airways, smoker 093 |
| GSM252819 |
large airways, smoker 094 |
| GSM252820 |
large airways, smoker 095 |
| GSM252821 |
large airways, smoker 097 |
| GSM252822 |
large airways, smoker 098 |
| GSM252823 |
large airways, smoker 102 |
| GSM252824 |
large airways, smoker 103 |
| GSM252825 |
large airways, smoker 112 |
| GSM252826 |
large airways, smoker 114 |
| GSM252827 |
large airways, smoker 119 |
| GSM252828 |
small airways, COPD 008 |
| GSM252829 |
small airways, COPD 012 |
| GSM252830 |
small airways, COPD 014 |
| GSM252831 |
small airways, COPD 015 |
| GSM252832 |
small airways, COPD 020 |
| GSM252833 |
small airways, COPD 036 |
| GSM252834 |
small airways, COPD 044 |
| GSM252835 |
small airways, COPD 046 |
| GSM252836 |
small airways, COPD 059 |
| GSM252837 |
small airways, COPD 068 |
| GSM252838 |
small airways, COPD 074 |
| GSM252839 |
small airways, COPD 080 |
| GSM252840 |
small airways, COPD 094 |
| GSM252841 |
small airways, COPD 103 |
| GSM252842 |
small airways, early-COPD 001 |
| GSM252843 |
small airways, early-COPD 009 |
| GSM252844 |
small airways, early-COPD 012 |
| GSM252845 |
small airways, early-COPD 021 |
| GSM252846 |
small airways, early-COPD 037 |
| GSM252847 |
small airways, early-COPD 045 |
| GSM252848 |
small airways, early-COPD 053 |
| GSM252849 |
small airways, early-COPD 054 |
| GSM252850 |
small airways, early-COPD 064 |
| GSM252851 |
small airways, early-COPD 086 |
| GSM252852 |
small airways, early-COPD 114 |
| GSM252853 |
small airways, early-COPD 132 |
| GSM252854 |
small airways, early-COPD 145 |
| GSM252855 |
small airways, non-smoker 001 |
| GSM252856 |
small airways, non-smoker 002 |
| GSM252857 |
small airways, non-smoker 003 |
| GSM252858 |
small airways, non-smoker 004 |
| GSM252859 |
small airways, non-smoker 005 |
| GSM252860 |
small airways, non-smoker 006 |
| GSM252861 |
small airways, non-smoker 008 |
| GSM252862 |
small airways, non-smoker 013 |
| GSM252863 |
small airways, non-smoker 014 |
| GSM252864 |
small airways, non-smoker 015 |
| GSM252865 |
small airways, non-smoker 019 |
| GSM252866 |
small airways, non-smoker 021 |
| GSM252867 |
small airways, non-smoker 035 |
| GSM252868 |
small airways, smoker 001 |
| GSM252869 |
small airways, smoker 027 |
| GSM252870 |
small airways, smoker 033 |
| GSM252871 |
small airways, smoker 040 |
| GSM252872 |
small airways, smoker 041 |
| GSM252873 |
small airways, smoker 044 |
| GSM252874 |
small airways, smoker 048 |
| GSM252875 |
small airways, smoker 049 |
| GSM252876 |
small airways, smoker 057 |
| GSM252877 |
small airways, smoker 058 |
| GSM252878 |
small airways, smoker 069 |
| GSM252879 |
small airways, smoker 074 |
| GSM252880 |
small airways, smoker 093 |
| GSM252881 |
small airways, smoker 094 |
| GSM252882 |
small airways, smoker 095 |
| GSM252883 |
small airways, smoker 097 |
| GSM252884 |
small airways, smoker 098 |
| GSM252885 |
small airways, smoker 103 |
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| Relations |
| BioProject |
PRJNA108189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10006_RAW.tar |
1.8 Gb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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