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Status |
Public on Dec 19, 2008 |
Title |
small airways, non-smoker 002 |
Sample type |
RNA |
|
|
Source name |
airway epithelial cells obtained by bronchoscopy and brushing
|
Organism |
Homo sapiens |
Characteristics |
age: 61 sex: M ethnic group: white smoking status: non-smoker
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
Description |
Comparison of gene expression in airway epithelial cells of normal non-smokers, phenotypic normal smokers, smokers with early COPD, and smokers with COPD.
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
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|
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Submission date |
Dec 21, 2007 |
Last update date |
May 29, 2009 |
Contact name |
Yael Strulovici-Barel |
E-mail(s) |
yas2003@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Department |
Department of Genetic Medicine
|
Lab |
Crystal
|
Street address |
1300 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE10006 |
Decreased Expression of Intelectin 1 in The Human Airway Epithelium of Smokers Compared to Nonsmokers |
GSE13933 |
Trachea Epithelium as a “ Canary” for Cigarette Smoking-induced Biologic Phenotype of Small Airway Epithelium |
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