|
| Status |
Public on Nov 12, 2019 |
| Title |
Rictorfl/fl LysM+/cre Colon_Macrophages_#4 |
| Sample type |
RNA |
| |
|
| Source name |
Rictorfl/fl LysM+/cre mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Colon
cell type: Macrophage
genotype/variation: Rictorfl/fl LysM+/cre
|
| Treatment protocol |
Mice were treated with 5%DSS for 5 days. After another 3 days of normal water, colon was isolated and macrophages were isolated.
|
| Growth protocol |
In order to induce colitis, mice were treated with 5%DSS for 5 days, followed by 3 days of normal water. Colon of the mice was digested and macrophages were sorted from the lamina propria cells. Macrophages were defined as being CD45+ CD11b+ CD64+ MHCII+ Ly6C-.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Colon macrophages were taken up in Trizol and RNA was isolated by phenol-chloroform extraction, followed by Quiagen Rneasy Plus Micro Kit according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with Affymetrix GeneChip® Mouse Gene 2.0 ST arrays
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
MJ_05042016_KATH_7
|
| Data processing |
Normalization of the data was done with the Affymetrix Expression Console. Analysis was performed with the Affymetrix Transcriptome Analysis Console (TAC) v3.0. We considered genes to be differentially regulated between Rictorfl/fl LysM+/+ and Rictorfl/fl LysM+/cre Colon macropahges that were more than two-fold differently expressed with a ANOVA significance value of smaller than 0.05.
|
| |
|
| Submission date |
Mar 13, 2017 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Thomas Weichhart |
| E-mail(s) |
thomas.weichhart@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Center for Pathobiochemistry and Genetics
|
| Street address |
Waehringerstraße 10
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE96525 |
Inactivation of mTORC2 in macrophages is a signature of colorectal cancer that promotes tumorigenesis. |
|
