|
| Status |
Public on Mar 14, 2018 |
| Title |
wt_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
wt, microglia cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
tissue: Brain
cell type: Microglial cell
gender: female
age: 24-28 weeks
genotype: Wildtype
|
| Treatment protocol |
All animals were injected with 8 mg Tamoxifen (20mg/ml Tamoxifen in corn oil) at 6 weeks of age to induce Hdac1 and 2 knock out in microglia.
|
| Growth protocol |
All animals were maintained in SPF condition
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Animals were killed by transcardial perfusion in anasthesia. Microglia cells were isolated from the brain by FACS sorting. Total RNA was extracted from sorted microglia by Pico Pure RNA extraction kit (Life Technologies) according to manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.0 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Scan protocol |
Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.0 ST Arrays , that were stained, washed and scanned following standard procedures.
|
| Description |
A977_03_588
|
| Data processing |
Background correction and normalization was performed using RMA as implemented in BRB-Tools
|
| |
|
| Submission date |
Mar 15, 2017 |
| Last update date |
Mar 14, 2018 |
| Contact name |
Ori Staszewski |
| E-mail(s) |
ori.staszewski@uniklinik-freiburg.de
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Institute of Neuropathology
|
| Street address |
Breisacher Str. 64
|
| City |
Freiburg |
| ZIP/Postal code |
D-79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE96622 |
Expression data from Hdac1 and 2 knock out microglia in 5X familial Alzheimer's disease (FAD) background |
| GSE98823 |
Knockout of HDAC1 and HDAC2 in Microglia |
|
