|
| Status |
Public on Oct 07, 2017 |
| Title |
A15066 |
| Sample type |
RNA |
| |
|
| Source name |
RNA, liver necropsy
|
| Organism |
Macaca mulatta |
| Characteristics |
animal#: A15066
tissue: liver
batch: 2
treatment: Uninfected infant
treatment 1: Uninfected_B_I
treatment 2: Uninfected_I
treatment 3: Uninfected
|
| Treatment protocol |
Microarray analysis was performed on post-necropsy liver samples on 31 animals. (11 adults and 20 infants). Of the adults 4 were uninfected, 5 SIV-infected, and 2 SIV-infected + cART treatment). Of the infants, 7 were uninfected, 9 SIV-infected, and 4 SIV-infected + cART treatment.
|
| Growth protocol |
31 Indian-orgin Rhesus Macaques were used. 11 adults (4 uninfected, 5 SIV-infected, 2 SIV-infected + cART treatment) and 20 infants (7 uninfected, 9 SIV-infected, 4 SIV-infected + cART treatment).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen liver powder obtained from necropsies using the NucleoSpin RNA isolation kit (Macherey-Nagel)
|
| Label |
Cy3
|
| Label protocol |
The Agilent One-Color Microarray-Based Gene Expression Analysis Protocol was followed for all processing steps, including Cy3-cDNA probe preparation, hybridization and array washing.
|
| |
|
| Hybridization protocol |
The Agilent One-Color Microarray-Based Gene Expression Analysis Protocol was followed for all processing steps, including Cy3-cDNA probe preparation, hybridization and array washing.
|
| Scan protocol |
Agilent Rhesus (8x60K) oligonucleotide microarrays were used. Dry slides were scanned on an Agilent Technologies Scanner G2505C US93503719 using the XDR setting.
|
| Data processing |
Raw images were analyzed using the Agilent Feature Extraction software (version 10.7.3.1) and the GE1-v5_95_Feb07 extraction protocol.
|
| |
|
| Submission date |
Apr 12, 2017 |
| Last update date |
Oct 08, 2017 |
| Contact name |
Michael Gale, Jr |
| E-mail(s) |
uw_galelab_geo@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Immunology
|
| Street address |
750 Republican St. E360, Box 358059
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17465 |
| Series (1) |
| GSE97676 |
Liver Macrophage Infiltration and Inflammation are associated with Antiviral Responses During SIV Infection in Macaques that is Only Partly Reduced during cART |
|
