|
| Status |
Public on Jan 23, 2008 |
| Title |
media, biol rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
HBE stimulated with media
|
| Organism |
Homo sapiens |
| Characteristics |
Primary HBEs
|
| Treatment protocol |
Cells were stimulated with media, or 20ng/mL human recombinant IL-22, or 10ng/mL hu recombinant IL-17, or both cytokines for 24 hrs
|
| Growth protocol |
Primary HBE cells were grown on semi permeable filters, were polarized and well differentiated and grown at air liquid interface
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Semipermeable membranes were placed in trizol, and trizol extraction of total RNA was performed
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Ambion TotalPrep kit from 395 ng total RNA according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 6.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome 133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 with 7G upgrade
|
| Description |
gene expression data - HBEs stim with media
|
| Data processing |
RMA (log2-transformed)
|
| |
|
| Submission date |
Jan 23, 2008 |
| Last update date |
Jan 23, 2008 |
| Contact name |
Shean Aujla |
| E-mail(s) |
shean.aujla@chp.edu
|
| Phone |
412 692 7338
|
| Organization name |
Children's Hospital of Pittsburgh
|
| Department |
Division of Pulmonary Medicine, Allergy and Immunology
|
| Lab |
Kolls Lab of Lung Immunology and Host Defense
|
| Street address |
3460 Fifth Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE10240 |
Primary Human Bronchial Epithelial Cells (HBEs) Stimulated with IL-22 and IL-17 |
|
