|
| Status |
Public on Nov 09, 2018 |
| Title |
prim-272-CD44- |
| Sample type |
RNA |
| |
|
| Source name |
primary-CRC272
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: colorectal cancer
tissue (system): primary
fraction (sorted cells): CD44- non-CSCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Target populations ranging in number from 5000 cells were double-sorted directly to Trizol reagent (Life Technologies). Total RNA extracted in accordance with the manufacture's instruction.
|
| Label |
Cy3
|
| Label protocol |
Cyanine3-labeled cRNA from each sample was prepared to mRNA amplification by using Low Input Quick Amp Labeling Kit (one-color) (Agilent Technologies) according to the manufacture's instruction.
|
| |
|
| Hybridization protocol |
cRNA (800 ng) was hybridized to the microarray chip (SurePrint G3 Human Gene Expression 8x60K v2, Agilent Technologies).
|
| Scan protocol |
Slides were scanned immediately after washing on the SureScan Microarray Scanner (G4900DA) using one color scan setting for 8x60k array slides (Scan resolution 3um, Dye channel is set to Green and Green PMT sensitivity is set to 100%).
|
| Description |
Gene expression of primary CRCs' CD44- non-CSCs
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Raw expression data was imported and normalized by using GeneSpring GX software (Agilent Technologies)
|
| |
|
| Submission date |
Jun 23, 2017 |
| Last update date |
Nov 09, 2018 |
| Contact name |
Michitaka Nakano |
| Organization name |
Kyushu University
|
| Department |
Department of Medicine and Biosystemic Sciences
|
| Street address |
3-1-1, Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE100433 |
Identification of gene signature in human colorectal CD44+ cancer stem cells |
|
