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Sample GSM27047 Query DataSets for GSM27047
Status Public on Jul 30, 2004
Title CONT. IP Experiment 1 Replicate 2
Sample type RNA
 
Channel 1
Source name Reference oligonucleotide set
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Labeled microRNA
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description 293 cells were tranfected with Myc-tagged argonaute family members and associated microRNAs quantitated by microarray analysis of immunoprecipitates. Human argonaute 1, 2, and 3, and a control immunoprecipitation were done. Two independent immunoprecipitations were performed, with two replicate points per immunoprecipitation. Ch1and Ch2 net values are median intensity, background subtracted, unnormalized. Normalized Ch2 values for argonaute immunoprecipitations are median center normalized. Normalized Ch2 values for control immunoprecipitates are normalized by a constant that is the average of the constants used for argonaute immunoprecipitates.
Keywords = microRNA
Keywords = miRNA
Keywords = argonaute
 
Submission date Jul 20, 2004
Last update date May 27, 2005
Contact name Scott M Hammond
E-mail(s) hammond@med.unc.edu
Phone 919-843-2366
Fax 919-966-1856
Organization name University of North Carolina
Department Dept Cell and Developmental Biology
Street address
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platform ID GPL1351
Series (1)
GSE1573 MicroRNA profiles of RISC sub-complexes

Data table header descriptions
ID_REF
VALUE Median Centered Normalized Log Ratio Values CH2/CH1
CH1_NET Background subtracted CH1 (635) Median Intensity
CH2_NET Background subtracted CH2 (532) Median Intensity
CH2_NORMALIZED NET
FLAG Denotes which features met our filtering criterion. A negative value means that the CH1_NET and CH2_NORMALIZED_NET were both below 1.5 Times Background

Data table
ID_REF VALUE CH1_NET CH2_NET CH2_NORMALIZED NET FLAG
1 -3.376345223 543 21 52.29 0
2 -5.258258567 953 10 24.9 0
3 -4.420256189 853 16 39.84 0
4 -4.338490286 806 16 39.84 0
5 -5.313813759 14361 145 361.05 0
6 -5.857980995 58 1 1 0
7 -4.135662635 12080 276 687.24 0
8 -5.082360449 6074 72 179.28 0
9 -5.47588838 7868 71 176.79 0
10 -4.35061085 254 5 12.45 0
11 -5.9412421 765 5 12.45 0
12 -5.448725848 435 4 9.96 0
13 -2.999209699 219 11 27.39 0
14 -3.919070719 339 9 22.41 0
15 -4.760669855 135 2 4.98 0
16 -4.760669855 135 2 4.98 0
17 -4.2837671 97 2 4.98 0
18 -3.558323376 88 3 7.47 0
19 -3.525156512 86 3 7.47 0
20 -4.012977854 201 5 12.45 0

Total number of rows: 156

Table truncated, full table size 4 Kbytes.




Supplementary data files not provided
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