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| Status |
Public on Jul 30, 2004 |
| Title |
MicroRNA profiles of RISC sub-complexes |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
293 cells were tranfected with Myc-tagged argonaute family members and associated microRNAs quantitated by microarray analysis of immunoprecipitates. Human argonaute 1, 2, and 3, and a control immunoprecipitation were done. Two independent immunoprecipitations were performed, with two replicate points per immunoprecipitation. Ch1and Ch2 net values are median intensity, background subtracted, unnormalized. Normalized Ch2 values for argonaute immunoprecipitations are median center normalized. Normalized Ch2 values for control immunoprecipitates are normalized by a constant that is the average of the constants used for argonaute immunoprecipitates.
Keywords = microRNA
Keywords = miRNA
Keywords = argonaute
Keywords = RNAi
Keywords: repeat sample
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| Contributor(s) |
Liu J, Carmell MA, Rivas FV, Marsden CG, Thomson JM, Song J, Hammond SM, Joshua-Tor L, Hannon GJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 20, 2004 |
| Last update date |
Mar 15, 2012 |
| Contact name |
Scott M Hammond |
| E-mail(s) |
hammond@med.unc.edu
|
| Phone |
919-843-2366
|
| Fax |
919-966-1856
|
| Organization name |
University of North Carolina
|
| Department |
Dept Cell and Developmental Biology
|
| Street address |
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| GSM27046 |
CONT. IP Experiment 1 Replicate 1 |
| GSM27047 |
CONT. IP Experiment 1 Replicate 2 |
| GSM27048 |
CONT. IP Experiment 2 Replicate 1 |
| GSM27049 |
CONT. IP Experiment 2 Replicate 2 |
| GSM27050 |
Ago1 IP Experiment 1 Replicate 1 |
| GSM27051 |
Ago1 IP Experiment 1 Replicate 2 |
| GSM27052 |
Ago1 IP Experiment 2 Replicate 1 |
| GSM27053 |
Ago1 IP Experiment 2 Replicate 2 |
| GSM27054 |
Ago2 IP Experiment 1 Replicate 1 |
| GSM27055 |
Ago2 IP Experiment 1 Replicate 2 |
| GSM27056 |
Ago2 IP Experiment 2 Replicate 1 |
| GSM27057 |
Ago2 IP Experiment 2 Replicate 2 |
| GSM27058 |
Ago3 IP Experiment 1 Replicate 1 |
| GSM27059 |
Ago3 IP Experiment 1 Replicate 2 |
| GSM27060 |
Ago3 IP Experiment 2 Replicate 1 |
| GSM27061 |
Ago3 IP Experiment 2 Replicate 2 |
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| Relations |
| BioProject |
PRJNA90337 |
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