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Sample GSM271149 Query DataSets for GSM271149
Status Public on Mar 06, 2008
Title NHE3+/+ replicate 3
Sample type RNA
 
Source name colon
Organism Mus musculus
Characteristics Genotype: wild-type
Age: 6-8 weeks
Treatment protocol N/A
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC to GeneChip® Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from the colon of wild-type mice
whole colonic tissue was dissected from mice 6-8 weeks of age
Data processing The data were initially analyzed with Afymetrix GCOS software using Affymetrix default analysis settings and global scaling as normalization method. Subsequently, data were imported to GeneSpring v.7.3.1 (Agilent).
 
Submission date Mar 05, 2008
Last update date Aug 28, 2018
Contact name Pawel R. Kiela
E-mail(s) pkiela@peds.arizona.edu
Phone 520-626-9687
Organization name University of Arizona
Department Pediatrics
Lab Gastroenterology and Nutrition
Street address 1501 N. Campbell Ave.
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
 
Platform ID GPL1261
Series (1)
GSE10740 Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCOS raw signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 773.4 P 0.000169
AFFX-BioB-M_at 1036.8 P 4.4e-05
AFFX-BioB-3_at 612.1 P 5.2e-05
AFFX-BioC-5_at 1998.1 P 4.4e-05
AFFX-BioC-3_at 2186.3 P 4.4e-05
AFFX-BioDn-5_at 3701 P 4.4e-05
AFFX-BioDn-3_at 7311.8 P 4.4e-05
AFFX-CreX-5_at 23674.6 P 5.2e-05
AFFX-CreX-3_at 29515.2 P 4.4e-05
AFFX-DapX-5_at 4.1 A 0.51489
AFFX-DapX-M_at 40 A 0.354453
AFFX-DapX-3_at 4 A 0.953518
AFFX-LysX-5_at 2.5 A 0.860518
AFFX-LysX-M_at 8.4 A 0.9273
AFFX-LysX-3_at 43 P 0.046467
AFFX-PheX-5_at 1.6 A 0.916408
AFFX-PheX-M_at 2.7 A 0.937087
AFFX-PheX-3_at 35.5 A 0.205732
AFFX-ThrX-5_at 17.7 A 0.737173
AFFX-ThrX-M_at 15 A 0.672935

Total number of rows: 45101

Table truncated, full table size 1214 Kbytes.




Supplementary file Size Download File type/resource
GSM271149.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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