|
Status |
Public on Mar 06, 2008 |
Title |
NHE3-/- replicate 2 |
Sample type |
RNA |
|
|
Source name |
colon
|
Organism |
Mus musculus |
Characteristics |
Genotype: NHE3-/- Age: 6-8 weeks
|
Treatment protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC to GeneChip® Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Description |
Gene expression data from the colon of NHE3-deficient mice whole colonic tissue was dissected from mice 6-8 weeks of age
|
Data processing |
The data were initially analyzed with Afymetrix GCOS software using Affymetrix default analysis settings and global scaling as normalization method. Subsequently, data were imported to GeneSpring v.7.3.1 (Agilent).
|
|
|
Submission date |
Mar 05, 2008 |
Last update date |
Aug 28, 2018 |
Contact name |
Pawel R. Kiela |
E-mail(s) |
pkiela@peds.arizona.edu
|
Phone |
520-626-9687
|
Organization name |
University of Arizona
|
Department |
Pediatrics
|
Lab |
Gastroenterology and Nutrition
|
Street address |
1501 N. Campbell Ave.
|
City |
Tucson |
State/province |
AZ |
ZIP/Postal code |
85724 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE10740 |
Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice |
|
Relations |
Reanalyzed by |
GSE119085 |