|
| Status |
Public on Mar 06, 2008 |
| Title |
NHE3-/- replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
colon
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: NHE3-/-
Age: 6-8 weeks
|
| Treatment protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 701021 Rev. 5, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC to GeneChip® Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from the colon of NHE3-deficient mice
whole colonic tissue was dissected from mice 6-8 weeks of age
|
| Data processing |
The data were initially analyzed with Afymetrix GCOS software using Affymetrix default analysis settings and global scaling as normalization method. Subsequently, data were imported to GeneSpring v.7.3.1 (Agilent).
|
| |
|
| Submission date |
Mar 05, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Pawel R. Kiela |
| E-mail(s) |
pkiela@peds.arizona.edu
|
| Phone |
520-626-9687
|
| Organization name |
University of Arizona
|
| Department |
Pediatrics
|
| Lab |
Gastroenterology and Nutrition
|
| Street address |
1501 N. Campbell Ave.
|
| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85724 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10740 |
Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
