|
| Status |
Public on Dec 26, 2017 |
| Title |
B10-52_52-G5-28274-14 [RhCMV] |
| Sample type |
SRA |
| |
|
| Source name |
Whole Blood
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: whole blood
monkey id: Rh28274
timepoint_postchallenge: 14
study: Study 1
harmonized disease score: 13
vaccine group: BCG_RhCMV.MTB.68_1_9Ag
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Paxgene RNA
GlobinClear Globin transcript reduction followed by Illumina mRNA-Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
processed data file:
rawCounts_GeneNames_AllSamples_RhCMV_mTB_1.csv
|
| Data processing |
Alignment STAR version STAR_2.3.1d
Sequenced reads were filtered for low-quality sequence and the presence of 'N's, then mapped to MacaM assembly using star STAR_2.3.1d
Gene counts were calculated by using HTSeq count
Genome_build: MacaM assembly
Supplementary_files_format_and_content: tab delimited file including the raw read counts for all the detected genes
|
| |
|
| Submission date |
Aug 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Daniel Zak |
| E-mail(s) |
danielzak000@gmail.com
|
| Organization name |
2Seventy Bio
|
| Lab |
Data Sciences
|
| Street address |
188 East Blaine Street, Suite 300
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98102 |
| Country |
USA |
| |
|
| Platform ID |
GPL14954 |
| Series (1) |
| GSE102440 |
Prevention of tuberculosis in rhesus macaques by a cytomegalovirus-based vaccine |
|
| Relations |
| BioSample |
SAMN07486579 |
| SRA |
SRX3081649 |
